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human his tagged adam10  (R&D Systems)


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    R&D Systems human his tagged adam10
    a Top: showing schematic and location of potential <t>ADAM10</t> cleavage sites in huFasL. Bottom: RhFasL and huFasL were incubated with ADAM10 for indicated times followed by immunoblotting (reducing condition) with anti-FasL antibody. Representative blot is n = 1. b RhFasL and huFasL were incubated with plasmin (Pln) for indicated times followed by immunoblotting (reducing condition) for FasL and plasmin. Representative blot is n = 1, however it has been repeated multiple times in manuscript with additional parameters. c Schematic of genetic construction of his-tagged 1X and 2XFasL constructs described in ( d ). d Indicated RhFasL and huFasL were incubated with Pln for O/N followed by immunoblotting for FasL. Representative blot is n = 1. e The kinetic binding behavior of huFasL and RhFasL was analyzed against fixed and immobilized human plasmin at indicated pH values. f Schematic of myc-tagged membrane attached FasL ECDs. g Indicated membrane FasL-expressing cells were treated with Pln followed by immunoblotting against c-myc. Representative blot is n = 1. h Cell survival assay after HuFasL/RhFasL ± Pln treatment ( n = 3, three independent experimental repeats) Untreated vs. huFasL, **** p = <0.0001; Untreated vs. huFasL+Pln, * p = 0.0214; Untreated vs. RhFasL, **** p = <0.0001; Untreated vs. RhFasL+Pln, **** p = <0.0001 < 0.0001; Unpaired, two-sided parametric t-test with no adjustments. i Same as ( h ), except lysates were analyzed for caspase-8 and PARP. Representative blot is n = 1, however it has been repeated in vivo with additional parameters, see Figs. , . Error bars in ( h ) are presented as mean ± standard deviation (SD). In ( i ) u.c. indicates uncleaved, c. indicates cleaved.
    Human His Tagged Adam10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human his tagged adam10/product/R&D Systems
    Average 93 stars, based on 36 article reviews
    human his tagged adam10 - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Evolutionary regulation of human Fas ligand (CD95L) by plasmin in solid cancer immunotherapy"

    Article Title: Evolutionary regulation of human Fas ligand (CD95L) by plasmin in solid cancer immunotherapy

    Journal: Nature Communications

    doi: 10.1038/s41467-025-60990-0

    a Top: showing schematic and location of potential ADAM10 cleavage sites in huFasL. Bottom: RhFasL and huFasL were incubated with ADAM10 for indicated times followed by immunoblotting (reducing condition) with anti-FasL antibody. Representative blot is n = 1. b RhFasL and huFasL were incubated with plasmin (Pln) for indicated times followed by immunoblotting (reducing condition) for FasL and plasmin. Representative blot is n = 1, however it has been repeated multiple times in manuscript with additional parameters. c Schematic of genetic construction of his-tagged 1X and 2XFasL constructs described in ( d ). d Indicated RhFasL and huFasL were incubated with Pln for O/N followed by immunoblotting for FasL. Representative blot is n = 1. e The kinetic binding behavior of huFasL and RhFasL was analyzed against fixed and immobilized human plasmin at indicated pH values. f Schematic of myc-tagged membrane attached FasL ECDs. g Indicated membrane FasL-expressing cells were treated with Pln followed by immunoblotting against c-myc. Representative blot is n = 1. h Cell survival assay after HuFasL/RhFasL ± Pln treatment ( n = 3, three independent experimental repeats) Untreated vs. huFasL, **** p = <0.0001; Untreated vs. huFasL+Pln, * p = 0.0214; Untreated vs. RhFasL, **** p = <0.0001; Untreated vs. RhFasL+Pln, **** p = <0.0001 < 0.0001; Unpaired, two-sided parametric t-test with no adjustments. i Same as ( h ), except lysates were analyzed for caspase-8 and PARP. Representative blot is n = 1, however it has been repeated in vivo with additional parameters, see Figs. , . Error bars in ( h ) are presented as mean ± standard deviation (SD). In ( i ) u.c. indicates uncleaved, c. indicates cleaved.
    Figure Legend Snippet: a Top: showing schematic and location of potential ADAM10 cleavage sites in huFasL. Bottom: RhFasL and huFasL were incubated with ADAM10 for indicated times followed by immunoblotting (reducing condition) with anti-FasL antibody. Representative blot is n = 1. b RhFasL and huFasL were incubated with plasmin (Pln) for indicated times followed by immunoblotting (reducing condition) for FasL and plasmin. Representative blot is n = 1, however it has been repeated multiple times in manuscript with additional parameters. c Schematic of genetic construction of his-tagged 1X and 2XFasL constructs described in ( d ). d Indicated RhFasL and huFasL were incubated with Pln for O/N followed by immunoblotting for FasL. Representative blot is n = 1. e The kinetic binding behavior of huFasL and RhFasL was analyzed against fixed and immobilized human plasmin at indicated pH values. f Schematic of myc-tagged membrane attached FasL ECDs. g Indicated membrane FasL-expressing cells were treated with Pln followed by immunoblotting against c-myc. Representative blot is n = 1. h Cell survival assay after HuFasL/RhFasL ± Pln treatment ( n = 3, three independent experimental repeats) Untreated vs. huFasL, **** p = <0.0001; Untreated vs. huFasL+Pln, * p = 0.0214; Untreated vs. RhFasL, **** p = <0.0001; Untreated vs. RhFasL+Pln, **** p = <0.0001 < 0.0001; Unpaired, two-sided parametric t-test with no adjustments. i Same as ( h ), except lysates were analyzed for caspase-8 and PARP. Representative blot is n = 1, however it has been repeated in vivo with additional parameters, see Figs. , . Error bars in ( h ) are presented as mean ± standard deviation (SD). In ( i ) u.c. indicates uncleaved, c. indicates cleaved.

    Techniques Used: Incubation, Western Blot, Construct, Binding Assay, Membrane, Expressing, Clonogenic Cell Survival Assay, In Vivo, Standard Deviation



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    human his tagged adam10  (R&D Systems)
    93
    R&D Systems human his tagged adam10
    a Top: showing schematic and location of potential <t>ADAM10</t> cleavage sites in huFasL. Bottom: RhFasL and huFasL were incubated with ADAM10 for indicated times followed by immunoblotting (reducing condition) with anti-FasL antibody. Representative blot is n = 1. b RhFasL and huFasL were incubated with plasmin (Pln) for indicated times followed by immunoblotting (reducing condition) for FasL and plasmin. Representative blot is n = 1, however it has been repeated multiple times in manuscript with additional parameters. c Schematic of genetic construction of his-tagged 1X and 2XFasL constructs described in ( d ). d Indicated RhFasL and huFasL were incubated with Pln for O/N followed by immunoblotting for FasL. Representative blot is n = 1. e The kinetic binding behavior of huFasL and RhFasL was analyzed against fixed and immobilized human plasmin at indicated pH values. f Schematic of myc-tagged membrane attached FasL ECDs. g Indicated membrane FasL-expressing cells were treated with Pln followed by immunoblotting against c-myc. Representative blot is n = 1. h Cell survival assay after HuFasL/RhFasL ± Pln treatment ( n = 3, three independent experimental repeats) Untreated vs. huFasL, **** p = <0.0001; Untreated vs. huFasL+Pln, * p = 0.0214; Untreated vs. RhFasL, **** p = <0.0001; Untreated vs. RhFasL+Pln, **** p = <0.0001 < 0.0001; Unpaired, two-sided parametric t-test with no adjustments. i Same as ( h ), except lysates were analyzed for caspase-8 and PARP. Representative blot is n = 1, however it has been repeated in vivo with additional parameters, see Figs. , . Error bars in ( h ) are presented as mean ± standard deviation (SD). In ( i ) u.c. indicates uncleaved, c. indicates cleaved.
    Human His Tagged Adam10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human his tagged adam10/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    human his tagged adam10 - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

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    a Top: showing schematic and location of potential ADAM10 cleavage sites in huFasL. Bottom: RhFasL and huFasL were incubated with ADAM10 for indicated times followed by immunoblotting (reducing condition) with anti-FasL antibody. Representative blot is n = 1. b RhFasL and huFasL were incubated with plasmin (Pln) for indicated times followed by immunoblotting (reducing condition) for FasL and plasmin. Representative blot is n = 1, however it has been repeated multiple times in manuscript with additional parameters. c Schematic of genetic construction of his-tagged 1X and 2XFasL constructs described in ( d ). d Indicated RhFasL and huFasL were incubated with Pln for O/N followed by immunoblotting for FasL. Representative blot is n = 1. e The kinetic binding behavior of huFasL and RhFasL was analyzed against fixed and immobilized human plasmin at indicated pH values. f Schematic of myc-tagged membrane attached FasL ECDs. g Indicated membrane FasL-expressing cells were treated with Pln followed by immunoblotting against c-myc. Representative blot is n = 1. h Cell survival assay after HuFasL/RhFasL ± Pln treatment ( n = 3, three independent experimental repeats) Untreated vs. huFasL, **** p = <0.0001; Untreated vs. huFasL+Pln, * p = 0.0214; Untreated vs. RhFasL, **** p = <0.0001; Untreated vs. RhFasL+Pln, **** p = <0.0001 < 0.0001; Unpaired, two-sided parametric t-test with no adjustments. i Same as ( h ), except lysates were analyzed for caspase-8 and PARP. Representative blot is n = 1, however it has been repeated in vivo with additional parameters, see Figs. , . Error bars in ( h ) are presented as mean ± standard deviation (SD). In ( i ) u.c. indicates uncleaved, c. indicates cleaved.

    Journal: Nature Communications

    Article Title: Evolutionary regulation of human Fas ligand (CD95L) by plasmin in solid cancer immunotherapy

    doi: 10.1038/s41467-025-60990-0

    Figure Lengend Snippet: a Top: showing schematic and location of potential ADAM10 cleavage sites in huFasL. Bottom: RhFasL and huFasL were incubated with ADAM10 for indicated times followed by immunoblotting (reducing condition) with anti-FasL antibody. Representative blot is n = 1. b RhFasL and huFasL were incubated with plasmin (Pln) for indicated times followed by immunoblotting (reducing condition) for FasL and plasmin. Representative blot is n = 1, however it has been repeated multiple times in manuscript with additional parameters. c Schematic of genetic construction of his-tagged 1X and 2XFasL constructs described in ( d ). d Indicated RhFasL and huFasL were incubated with Pln for O/N followed by immunoblotting for FasL. Representative blot is n = 1. e The kinetic binding behavior of huFasL and RhFasL was analyzed against fixed and immobilized human plasmin at indicated pH values. f Schematic of myc-tagged membrane attached FasL ECDs. g Indicated membrane FasL-expressing cells were treated with Pln followed by immunoblotting against c-myc. Representative blot is n = 1. h Cell survival assay after HuFasL/RhFasL ± Pln treatment ( n = 3, three independent experimental repeats) Untreated vs. huFasL, **** p = <0.0001; Untreated vs. huFasL+Pln, * p = 0.0214; Untreated vs. RhFasL, **** p = <0.0001; Untreated vs. RhFasL+Pln, **** p = <0.0001 < 0.0001; Unpaired, two-sided parametric t-test with no adjustments. i Same as ( h ), except lysates were analyzed for caspase-8 and PARP. Representative blot is n = 1, however it has been repeated in vivo with additional parameters, see Figs. , . Error bars in ( h ) are presented as mean ± standard deviation (SD). In ( i ) u.c. indicates uncleaved, c. indicates cleaved.

    Article Snippet: Recombinant HuFasL and RhFasL containing the extracellular FasL domain (aa 103–281) were incubated with recombinant human His-tagged ADAM10 (Catalog #: 936-AD, R&D Systems, USA) (1μ/20 μl reaction mixture) for indicated time at 37 °C in reaction buffer (25 mM Tris, 0.005% Brij-35; 2.5 μM ZnCl 2 , pH 8.8) as published earlier .

    Techniques: Incubation, Western Blot, Construct, Binding Assay, Membrane, Expressing, Clonogenic Cell Survival Assay, In Vivo, Standard Deviation